CHEM 4402
"Lab today? YES!!!"
All exercises are adapted from the Green Fluorescent Protein Biochemistry Laboratory Manual (copyright 2005 Patrick Larkin)
GFP Lab 1 - Literature searching
GFP Lab 2 - Database searching
GFP Lab 3 - PCR Primer Design
GFP Lab 4 - Polymerase Chain Reaction
GFP Lab 5 - Electrophoresis & quantitation of DNA
GFP Lab 6 - Ligation & Bacterial Transformation
GFP Lab 7 - Plasmid DNA Isolation & Restriction Enzyme Analysis (I)
GFP Lab 8 - Restriction Enzyme analysis (II) & DNA Sequencing (I)
GFP Lab 9 - DNA sequencing (II)
GFP Lab 10 - Protein Explorer
GFP Lab 11 - GFP Induction
GFP Lab 12 - Gel Permeation Chromatography
GFP Lab 13 - SDS-Polyacrylamide Gel electrophoresis